Study of Germination and Seedling Growth Parameters of Three Clover Species (Trifolium spp.) under Drought and Salinity Stresses

DOR: 98.1000/2383-1251.1398.6.145.11.1.1575.1610 Extended Abstract Introduction: Agriculture has been influenced by different abiotic stresses such as temperature, drought and salinity, which reduces roughly half of the yield of crops. In many forage plants, germination and early seedling growth are the most sensitive stages of their growth in the face of environmental stresses. Current research was conducted to study the effects of drought and salinity iso-osmosis stresses on germination indices and growth parameters of three clover species, including Trifolium resupinatum, T. alexandrinum and T. incarnatum. Material and Methods: Two separate experiments (drought and salinity) were conducted as a factorial experiment based on a completely randomized design with three replications. The test factors included clover species with three levels, including T. resupinatum, T. alexandrinum and T. incarnatum and different levels of drought and salinity potentials (0, -2, -4, -6, -8, -10 and -12 bar) due to polyethylene glycol 6000 (PEG 6000) and sodium chloride (NaCl), respectively. Sterile disposable petri dishes with a diameter of 10 cm were used, in each of which, 27 seeds were placed on filter paper and then 5 ml of the desired treatment solution was added to each. Petri dishes were then placed in a germinator at 20°C and relative humidity of 75%. After the end of the desired time germination (About 14 days), from each petri, germination percentage and rate, number of secondary roots, time to get 50% germination (D50), root to shoot ratio and the number of abnormal seedlings were recorded. Results: Inhibitory effects of salinity and drought stresses affected all germination indices. Germination percentage, rate of germination, root and shoot length all decreased with increasing stress levels, while time to get 50% germination (D50) increased with the increase in stress levels. Under both drought and salinity, all growth parameters of Trifolium resupinatum were higher than the other two species. Germination percentage of T. incarnatum was 0% at -12 bar of drought, but germination of T. resupinatum and T. alexandrinum was 70.33% and 7.33% at -12 bar of these stresses, respectively. Under salinity conditions, all the seeds of the three species germinated at -12 bar. Root to shoot ratio increased with increasing stress levels and at high stress levels, it decreased. The decreases were 41.39% and 0% in drought and salinity, respectively. The number of secondary roots increased with increasing stress levels and the maximum number was observed in Trifolium alexandrinum which was 5.42 and 1 in drought and salinity, respectively. The number of abnormal seedling increased with increasing salinity levels, while under drought conditions there was no abnormal seedling. Conclusion: Comparison of the effects of sodium chloride and polyethylene glycol showed that sodium chloride reduced germination index due to toxic effects or osmotic effects more than polyethylene glycol solution. ­­­­­­­­­­­­­­­­­­­It seems that Trifolium resupinatum has the best yield in both drought and salinity stresses, and that in drought condition, Trifolium alexandrinum, and in salinity stress, Trifolium incarnatum have the best performance.                                                                                                                               Highlights: Introduction of clover species with more tolerance to drought and salinity in germination stage. Evaluation of germination indices of clover species under drought and salinity iso-osmotic conditions.